Screening and identification of efficient lignolytic / cellulolytic microbes for effective decomposition of seri residue

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1.

Project Code

:

PRP- 3274

2.

Title

:

Screening and identification of efficient lignolytic / cellulolytic microbes for effective decomposition of seri residue.

 

3.

Investigators

:

Naik VN, Sharma DD, Chowdary NB

4.

Project period

:

October 2006 -September 2010

5.

Institute/ University

 

CSR&TI, Mysore

6.

Project cost

:

1.32 lakhs

7.

Objectives

  • To collect, isolate and identify the efficient lignolytic/cellulolytic microbes and testing their pathogenicity on mulberry.
  • To develop a bio-product from the identified microbes for effective decomposition of seri residue, especially left over mulberry shoots.

8.

Important findings

75 microbes (fungi and bacteria) were isolated from various sources (paper pulp, bagasse, dead and decaying wood).

They were tested for lignolytic and cellulolytic activity.

Genera like Pleurotus, Phanerochaete, Lentinus, Agaricus & Calocybe showed both lignolytic and cellulolytic activity. These microbes were found non pathogenic to mulberry. They were further screened for three enzymes associated with lignin degradation such as laccase, lignin peroxidase & Manganese peroxidase.  Two isolates, Pleurotus florida and P. ostreatus showed positive reaction for all the three enzymes and selected for d

ecomposition of mulberry shoots. 11 treatments in different combinations were imposed under anaerobic condition for hastening the decomposition of mulberry shoot.  

Treatment-9: Mulberry shoot + Single Super Phosphate + Lime powder + Sugarcane bagasse + Pleurotus florida was found effective in decomposing the mulberry shoot up to 92% withinin 150 days.

Chemical analysis of the decomposed mulberry shoot showed pH: 7.2; EC: <1, OC: 1.61 - 3.06%; N: 0.20 - 0.29 %; P: 0.11 - 0.22% and K: 0.18 - 0.32. It helps in hygienic disposal of seri-residue and increasing organic content (3.06%) in the soil.

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