Investigations on the association of fungal and bacterial pathogens with root knot nematode disease-complex and their management in mulberry

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1.

Project Code

:

PRP- 3273

2.

Title

:

Investigations on the association of fungal and bacterial pathogens with root knot nematode disease-complex and their management in mulberry

3.

Investigators

:

Naik VN, Govindaiah, Sharma DD, Chowdary NB.

4.

Project period

:

September 2002 - September 2005

5.

Institute/ Unversity

 

CSR&TI, Mysore

6.

Project cost

:

14.06 lakhs

7.

Objectives

  • T
  • To know the impact of interaction between root knot nematode and soil borne fungal pathogens at sequential and concomitant inoculation on disease severity and their effect on growth of mulberry.
  • To screen various bioagents, botanicals & oil cakes for developing an IDM strategy for root knot disease complex.

8.

Important findings

Fusarium solani and F. oxysporum were found associated with root knot and proved their pathogenicity on mulberry.

Interaction between nematode and virulent fungi (F. solani and F. oxysporum) revealed high disease incidence when nematode and two fungi present together resulting in maximum (88.9%) mortality.

A talc-based bio-consortium Trichoderma harzianum & T. viride developed with shelf life of 12 months at room (25-30°C) temperature.

Based on in vitro study, the effective botanical (Lawsonia inermis), Pongamia oil cake and mixed bio-nematofungicide were selected as treatments alone and in combination for IDM against disease complex.

Maximum reduction (root rot & egg masses) of disease complex was recorded when bio-consortium (Th-o + Tv) was mixed with Pongamia oil cake and FYM (1:10:25), which reduced the disease severity to 75-80%.

Recommendation: 1kg bio-consortium mixed with 10 kg Pongamia oil cake and 50 kg FYM (for 300 plants) is recommended.

The mixture is applied @ 200 g/plant (3 times/year at an interval of 4 months) followed by irrigation. The technology does not have any residual toxicity on mulberry and silkworms.

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